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A non-invasive and label-free technique, Raman spectroscopy is an ideal analytical tool for tissue analysis.
You can extract a full spectrum of chemical information (from entities such as nucleic acids, proteins and lipids) without the need for targeting biomolecules, markers, stains or dyes. Unlike many other analysis techniques—such as western blot, GC/MS, and MALDI-TOF—Raman analysis does not require the sample to be homogenized.
Quickly and accurately identify tissue layers
Distinguish, identify and demarcate pre-cancerous, cancerous and healthy tissues.
- No need for conjugation with antibodies: save both money and time when optimising protocols
- Reliably demarcate and objectively identify anatomical layers in the tissues, based on their total molecular composition. Delineate tumour margins
- Avoid subjective colourimetry and morphology-based analysis
- Identify chemical changes in the tissues that have not manifested themselves in morphological changes (e.g. levels of DNA/RNA, glycogen, lipid, protein, lipid phase and DNA integrity)
Understand biological systems
Get a complete chemical analysis of tissues and understand the underlying mechanism of their changes.
- the development of an organism
- the pathogenesis of diseases
- tissue's response to drugs or stimulants (e.g. chemotherapeutic agents, toxins and anti-inflammatories)
Examine in one procedure:
- changes in the level and conformation of proteins, lipids, nucleic acids and carbohydrates
- the presence of mineral deposits (e.g. calcification in breast tissue and atherosclerosis)
- the redox states of heme proteins (e.g. neuroglobins and myoglobins)
Renishaw's StreamLine™ technology is particularly suited to generating tissue images. Its line-focus geometry enables you to use high laser powers whilst avoiding photothermal damage to the tissue. This maximises signal levels and gives you images in the shortest possible time.
Study uneven surfaces
With StreamLine's Surface capability, you can generate images of samples, even if they are not flat.
Slide scanning automation
You can configure Renishaw's fully automated Raman systems to scan sequentially multiple histological sections and run unattended. This saves you time and enables you to maximise the productivity of your Raman system.
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To find out more about this application area, or an application that isn't covered here, contact our applications team.
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Downloads: life sciences (tissues)
Application note: Monitoring transdermal drug delivery in skin using the RA816 Biological Analyser
Confirming the presence of a topical compound in the epidermis and reticular dermis with high specificity and sensitivity.
Application note: Classification of brain glioma tumours using the Renishaw Biological Analyser
Demonstrating discrimination between diseased and healthy brain tissue using the Renishaw RA816 Biological Analyser.
Application note: Tissue imaging with the inVia Raman microscope
Raman tissue imaging is a unique method that can simultaneously describe the molecular composition and the distribution of multiple chemical species in tissues at a high spatial resolution, without labelling.
Application note: Raman imaging to reveal components and metabolites in wood cells and tissue
Analysing Scots pine wood using the inVia™ confocal Raman microscope, to reveal high-resolution details of structure and chemical composition.
Product note: Rayleigh imaging using the inVia™ confocal Raman microscope
Product note detailing how you can perform Rayleigh imaging on the inVia confocal Raman microscope.
Application note: Virsa Raman analyser for in vivo studies
The Virsa™ Raman analyser is ideal for developing clinical applications of Raman spectroscopy. It is available with a clinically-ready fibre-optic probe developed in partnership with EmVision, leaders in medical fibre probes.
You may be interested in these papers:
Kast et al (2014) J Neurooncol doi 10.1007/s11060-014-1536-9
Bonifacio et al (2010) Analyst 135: 3193-3204
Researchers used Renishaw RA816 Biological Analyser to identify the genetically different subtypes of brain gliomas in a surgical setting.
A multidisciplinary group of scientists and engineers are using Raman spectroscopy to understand the damage in cells and tissues caused by radiation used in cancer treatment.
The Children's Hospital of Michigan and Wayne State University use a Renishaw inVia confocal Raman microscope in the study of childhood diseases.